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img=cv2.imread("1.4301_20x_dunkel.png",0)
plt.imshow(img)
rows , cols=img.shape

s = np.linspace(0, 2*np.pi, 400)
x = 1250 +100*np.cos(s)
y = 750 + 100*np.sin(s)
init = np.array([x, y]).T



snake = active_contour(gaussian(img, 3),
                      init, alpha=-0.015, beta=10, gamma=0.001)

fig, ax = plt.subplots(figsize=(7, 7))
ax.imshow(img, cmap=plt.cm.gray)
ax.plot(init[:, 0], init[:, 1], '--r', lw=3)
ax.plot(snake[:, 0], snake[:, 1], '-b', lw=3)
ax.set_xticks([]), ax.set_yticks([])
ax.axis([0, img.shape[1], img.shape[0], 0])