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import wx.lib.plot as plot 

...


def plotting(self):
    for i in plots(nr):
        self.canvas = plot.PlotCanvas(self.panel) 
        self.vbox.Add(self.canvas, 1, wx.EXPAND | wx.ALL, 5) 

        lines = ( 
            plot.PolyLine( 
                coordinates1, colour = "blue", legend = u" Eff Hits", width = 2 
            ), 
            plot.PolyLine( 
                coordinates2, colour = "red", legend = u" All Hits", width = 2 
            ), 
        ) 

        graphics = plot.PlotGraphics( 
            objects = lines#, title = u"Test", xLabel = u"RNAi Query Length"
            , 
            yLabel = a 
        ) 
        self.canvas.Draw(graphics, xAxis = (0, int(seq)), yAxis = (0, 30)) 
...

def OnFilePrint(self, event):
    self.canvas.Printout()
    
...